The high abundance of ribosomal RNA (rRNA) in prokaryotes (> 85% of total RNA) limits detection efficiency of relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) or other analysis. The Pan-Prokaryote riboPOOL offers a universal and affordable solution to deplete rRNAs from a wide spectrum of bacteria and archaebacteria. Consisting of a highly complex mixture of biotinylated oligos, optimally designed for maximal coverage of major phyla, the Pan-Prokaryote riboPOOL can be applied to culture preparations, metagenomic or microbiome samples. The Pan-Prokaryote riboPOOL targets 5S, 16S and 23S rRNA, and is used together with streptavidin-coated magnetic beads to remove rRNAs efficiently. The Pan-Prokaryote riboPOOL is an affordable and versatile solution for the microbiology community and has been RNA-Seq validated for a growing list of species (next page).
- Broad microbial coverage, suited for Metagenomic/Microbiome analysis
- Highly efficient rRNA depletion
- Targets 5S, 16S and 23S rRNA
- Affordable, versatile solution