VIROMER®: A NOVEL POLYMER BASED TRANSFECTION REAGENT MIMICKING THE VIRAL INFECTION PROCESS BY AN ACTIVE ENDOSOME ESCAPE MECHANISM.
After binding RNA or DNA molecules, Viromer complexes enter the cell via endocytosis. Due to an active escape from the endosome a highly efficient delivery of molecules into the cytoplasma occurs. Once reached the cyctoplasma, Viromer complexes disaggregate – releasing RNA or DNA.
The innovative Viromer complexes are non-charged, preventing the aggregation with proteins which are included in fetal calf serum or other cell culture media supplements. This leads to the great advantage, that Viromer is very gentle on cells, compatible with serum, antibiotics and differentiation factors. In addition to this convenience, a magnificent benefit of working with Viromer is the possibility to transfect suspension cells by getting a high efficiency in siRNA or mRNA transfection as zero charged Viromer complexes do not aggregate with the negative charged cellsurface.